Canine Echinococcus Multilocularis ELISA Kit is sandwich-antibody enzyme linked immunosorbent assay designed to detect
the presence of antigen to canine Echinococcus Multilocularis
the canine stool. A microtitration format has been configured by
coating canine Echinococcus antibody in alternating columns on
the plate. Upon incubation of the test sample in the coated well,
antigens specific to canine Echinococcus Multilocularis forms a
complex with the coated viral antibodies. After washing away unbound
material from the wells, an anti-porcine horseradish peroxidase conjugate is added that binds to any canine
antigens attached in the wells. In the final step of the assay, unbound conjugate
is washed away, and enzyme substrate and a chromogen, TMB, are added to
the wells. Stop the reaction by adding stop solution. Within 15 minutes READ
the absorbance at 450 nm using 630 nm as the reference wavelength if available.
Enzyme immunoassays for the qualitative of antigens against canine Echinococcus
Multilocularis the stool samples.