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Acid Black 1 structure
Acid Black 1 structure

Acid Black 1

Iupac Name:disodium;4-amino-3-[(4-nitrophenyl)diazenyl]-5-oxo-6-(phenylhydrazinylidene)naphthalene-2,7-disulfonate
CAS No.: 1064-48-8
Molecular Weight:616.487
Modify Date.: 2023-02-13 22:14
Introduction: Used for staining proteins on polyacrylamide gel, agarose gel and nitrocellulose membrane.?After electrophoresis, it is recommended to fix the protein on the gel.?The glue is stained with 0.1% amino black 10B in 7% (v/v) acetic acid solution for at least 2 hours, and then decolorized with 7% (v/v) acetic acid solution.?The detection sensitivity is about 20% of the Coomassie Brilliant Blue R250. View more+
1. Names and Identifiers
1.1 Name
Acid Black 1
1.2 Synonyms

2,7-naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[(E)-(4-nitrophenyl)azo]-6-[(E)-phenylazo]-, disodium salt 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-[(E)-2-(4-nitrophenyl)diazenyl]-6-[(E)-2-phenyldiazenyl]-, sodium salt (1:2) 2,7-Naphthalenedisulfonic acid, 5-amino-3,4-dihydro-6-[(E)-2-(4-nitrophenyl)diazenyl]-4-oxo-3-(2-phenylhydrazinylidene)-, sodium salt, (3E)- (1:2) 2,7-naphthalenedisulfonicacid,4-amino-5-hydroxy-3-[(4-nitrophenyl)azo]-6-(phe 4-Amino-5-hydroxy-3-[(E)-(4-nitrophényl)diazènyl]-6-[(E)-phényldiazènyl]-2,7-naphtalènedisulfonate de disodium ACID BLACK acidblackh Amido Black Staining Amido Schwarz aniline blue black Black 10B CI NO 0470 DermBlueP Dinatrium-4-amino-5-hydroxy-3-[(E)-(4-nitrophenyl)diazenyl]-6-[(E)-phenyldiazenyl]-2,7-naphthalendisulfonat Disodium (3E)-5-amino-6-[(E)-(4-nitrophenyl)diazenyl]-4-oxo-3-(phenylhydrazono)-3,4-dihydro-2,7-naphthalenedisulfonate Disodium 4-amino-5-hydroxy-3-[(E)-(4-nitrophenyl)diazenyl]-6-[(E)-phenyldiazenyl]-2,7-naphthalenedisulfonate Disodium 4-amino-5-hydroxy-3-[(E)-(4-nitrophenyl)diazenyl]-6-[(E)-phenyldiazenyl]naphthalene-2,7-disulfonate KURO401 MFCD00004017 NAPHTHALENE BLACK 10B NAPHTHALENE BLACK B naphthol blue-black naphtol blue black

1.3 CAS No.
1.4 CID
280-105-8; 213-903-1; 616-852-7
1.6 Molecular Formula
C22H14N6Na2O9S2 (isomer)
1.7 Inchi
1.8 InChkey
1.9 Canonical Smiles
1.10 Isomers Smiles
2. Properties
2.1 Density
1.05 g/mL at 20 °C
2.1 Melting point
275 C
2.1 Refractive index
1.773 (Predicted)
2.1 Flash Point
2.1 Precise Quality
2.1 PSA
2.1 logP
2.1 Solubility
2.2 Λmax
2.3 Appearance
Dark red to black powder
2.4 Storage
Hygroscopic. Ambient temperatures.
2.5 Chemical Properties
red to maroon crystalline solid
2.6 Color/Form
2.7 PH
9.0 (10g/l, H2O, 25℃)
2.8 Water Solubility
10 G/L (25 oC)
2.9 Stability
Stable. Incompatible with strong oxidizing agents.
2.10 StorageTemp
Store at RT.
3. Use and Manufacturing
3.1 Definition
ChEBI: An organic sodium salt resulting from the formal condensation of Amido Black 10B (acid form) with two equivalents of sodium hydroxide.
3.2 General Description
Dark brown granular solid.
3.3 Usage

A stain for acidic peptides.

4. Safety and Handling
4.1 Symbol
GHS02, GHS07
4.1 Hazard Codes
4.1 Signal Word
4.1 Risk Statements
4.1 Safety Statements
4.1 Fire Hazard
Flash point data for Acid Black 1 are not available; however, Acid Black 1 is probably combustible. Acid Black 1 Preparation Products And Raw materials Raw materials
4.2 Hazard Declaration
4.2 Caution Statement
P261-P305 + P351 + P338
4.2 WGK Germany
4.2 Report

Reported in EPA TSCA Inventory.

4.3 Safety

Safety Information of Acid Black 1 (CAS NO.1064-48-8):
Hazard Codes:Xi? ,C? ,F
Risk Statements:36/37/38-10-34-11
36/37/38:? Irritating to eyes, respiratory system and skin?
34:Causes burns??
11:Highly Flammable
Safety Statements:26-36-37/39-45-36/37/39-23-16
26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
36:Wear suitable protective clothing
37/39:Wear suitable gloves and eye/face protection
45:In case of accident or if you feel unwell, seek medical advice immediately (show label where possible)
36/37/39:Wear suitable protective clothing, gloves and eye/face protection
23:Do not breathe gas/fumes/vapor/spray (appropriate wording to be specified by the manufacturer)
16:Keep away from sources of ignition - No smoking??????????
RIDADR:UN 2924 3/PG 3
WGK Germany:3
HS Code:32041200
Mutation data reported. When heated to decomposition it emits toxic vapors of NOx and SOx.

4.4 Specification

?Acid Black 1 , its CAS NO. is 1064-48-8, the synonyms are Name of Substance ; 2,7-Naphthalenedisulfonic acid, 4-amino-5-hydroxy-3-((4-nitrophenyl)azo)-6-(phenylazo)-, disodium salt ; 4-Amino-5-hydroxy-3-((4-nitrophenyl)azo)-6-(phenylazo)-2,7-naphth- alenedisulfonic acid, disodium salt ; Amido black .

4.5 Toxicity
1. ???

mmo-sat 50?μg/plate

??? EMMUEG ?? Environmental and Molecular Mutagenesis. 22 (1993),188.
2. ???

mma-sat 50?μg/plate

??? EMMUEG ?? Environmental and Molecular Mutagenesis. 22 (1993),188.

2.Hazard identification

2.1 Classification of the substance or mixture

Skin sensitization, Category 1B

Specific target organ toxicity \u2013 repeated exposure, Category 1

2.2 GHS label elements, including precautionary statements

Signal word


Hazard statement(s)

H317 May cause an allergic skin reaction

H372 Causes damage to organs through prolonged or repeated exposure

Precautionary statement(s)

P261 Avoid breathing dust/fume/gas/mist/vapours/spray.

P272 Contaminated work clothing should not be allowed out of the workplace.

P280 Wear protective gloves/protective clothing/eye protection/face protection.

P260 Do not breathe dust/fume/gas/mist/vapours/spray.

P264 Wash ... thoroughly after handling.

P270 Do not eat, drink or smoke when using this product.


P302+P352 IF ON SKIN: Wash with plenty of water/...

P333+P313 If skin irritation or rash occurs: Get medical advice/attention.

P321 Specific treatment (see ... on this label).

P362+P364 Take off contaminated clothing and wash it before reuse.

P314 Get medical advice/attention if you feel unwell.




P501 Dispose of contents/container to ...

2.3 Other hazards which do not result in classification


7. Other Information
7.0 Preparation
4-Nitrobenzenamine diazo, in acid conditions and 4-Amino-5-hydroxynaphthalene-2,7-disulfonic acid?coupling, aniline diazo, in alkaline conditions and formation of single accidentally coupled nitrogen compounds.
7.1 BRN
7.2 Chemical Properties
red to maroon crystalline solid
7.3 Definition
ChEBI: An organic sodium salt resulting from the formal condensation of Amido Black 10B (acid form) with two equivalents of sodium hydroxide.
7.4 General Description
Dark brown granular solid.
7.5 Air & Water Reactions
Can be explosive when as a dust in air at certain concentrations. Moderately soluble in water.
7.6 Reactivity Profile
Acid Black 1 may form toxic gases with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. May form flammable gases with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Reacts with iron to give a weaker, greener color .
7.7 Fire Hazard
Flash point data for Acid Black 1 are not available; however, Acid Black 1 is probably combustible.
7.8 Usage
Amido Black 10B is used as a histological, protein, and nucleic acid dye used in electrophoresis. Naphthol Blue Black is an important and commonly used industrial dye. This dye is also used in rapid staining of protein bands on nitrocellulose membranes such as Western blots. Naphthol Blue Black can be utilized as a nucleic acid stain in SDS-Page gels. Additionally this compound has been untilized in cell proliferation assays and histochemical studies. It is also used in criminal investigations to detect blood present with latent fingerprints. They are also used in paints, inks, plastics and leather.
7.9 Usage
Used to stain proteins in polyacrylamide gels.
8. Computational chemical data
  • Molecular Weight: 616.487g/mol
  • Molecular Formula: C22H14N6Na2O9S2
  • Compound Is Canonicalized: True
  • XLogP3-AA: null
  • Exact Mass: 616.00590696
  • Monoisotopic Mass: 616.00590696
  • Complexity: 1110
  • Rotatable Bond Count: 4
  • Hydrogen Bond Donor Count: 2
  • Hydrogen Bond Acceptor Count: 14
  • Topological Polar Surface Area: 273
  • Heavy Atom Count: 41
  • Defined Atom Stereocenter Count: 0
  • Undefined Atom Stereocenter Count: 0
  • Defined Bond Stereocenter Count: 0
  • Undefined Bond Stereocenter Count: 0
  • Isotope Atom Count: 0
  • Covalently-Bonded Unit Count: 3
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10. Realated Product Infomation