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Home> Encyclopedia >   /  Pharmaceutical Intermediates  /  Pharmaceutical  /  Organic Intermediate  /  Others  /  Pharmaceuticals and Biochemicals
Guanidine thiocyanate structure
Guanidine thiocyanate structure

Guanidine thiocyanate

CAS No.:593-84-0
Molecular Weight:118.16084
Introduction: Guanidine thiocyanate is one of the cheapest and easiest to prepare of the guanidine salts. Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity. View more+
1. Names and Identifiers
1.1 Name
Guanidine thiocyanate
1.2 Synonyms

Buffer AVL Buffer ML Buffer MTL Buffer RLT GTC GUANIDINE HYDROTHIOCYANATE GUANIDINE ISOTHIOCYANATE GUANIDINE MONOTHIOCYANATE GUANIDINE RHODANIDE Guanidine, monothiocyanate Guanidinium isothiocyanate GUANIDINIUM THIOCYANATE NSC 2119 RLT Thiocyanic acid, compd. with guanidine (1:1)

1.3 CAS No.
1.5 Molecular Formula
C2H6N4S (isomer)
1.6 Inchi
1.7 InChkey
1.8 Canonical Smiles
1.9 Isomers Smiles
2. Properties
3.1 Density
1.103 g/mL at 20 °C
3.1 Melting point
3.1 Boiling point
132.9 °C at 760 mmHg
3.1 Refractive index
n20/D 1.482
3.1 Flash Point
34.2 °C
3.1 Precise Quality
3.1 PSA
3.1 logP
3.1 Solubility
H2O: 6?M at?20?°C, clear, colorless
3.2 Appearance
white powder
3.3 Chemical Properties
White cryst.powder
3.4 Color/Form
White or colorless
3.5 Odor
3.6 PH
4.8-6.0 (1420g/l, H2O, 20℃)(saturated solution)
3.7 Water Solubility
H2O: 6 M at 20 °C, clear, colorless
3.8 Stability
Stability Stable, but light sensitive. Incompatible with acids (contact releases very toxic gas), strong oxidising agents.
3.9 StorageTemp
Store at RT.
3. Use and Manufacturing
4.1 Storage
Light Sensitive. Ambient temperatures.
4.2 Usage
Guanidine thiocyanate has been used:• for the lysis of colorectal adenocarcinoma cell line prior to RNA isolation• as a PCR inhibitor in triplex mitochondrial DNA (mtDNA) qPCR assay
4. Safety and Handling
5.1 Symbol
5.1 Hazard Codes
5.1 Signal Word
5.1 Risk Statements
5.1 Safety Statements
5.1 Packing Group
5.1 Hazard Class
5.1 Hazard Declaration
H302 + H312 + H332; H314; H412
5.1 WGK Germany
5.1 Safety
Hazard Codes:Xn,T
Risk Statements:20/21/22-32-52/53-36/37/38
20/21/22:Harmful by inhalation, in contact with skin and if swallowed
32:Contact with acids liberates very toxic gas
52/53:Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment
36/37/38:Irritating to eyes, respiratory system and skin
Safety Statements:36/37-61-13-36-26
36/37:Wear suitable protective clothing and gloves
61:Avoid release to the environment. Refer to special instructions safety data sheet
13:Keep away from food, drink and animal feeding stuffs
36:Wear suitable protective clothing
26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice
WGK Germany:2
HS Code:29252000
5.2 Specification

White cryst.powder
Safety Statements:36/37-61-13-36-26
36/37:Wear suitable protective clothing and gloves
61:Avoid release to the environment. Refer to special instructions safety data sheet
13:Keep away from food, drink and animal feeding stuffs
36:Wear suitable protective clothing
26:In case of contact with eyes, rinse immediately with plenty of water and seek medical advice

2.Hazard identification

2.1 Classification of the substance or mixture

Acute toxicity - Oral, Category 4

Acute toxicity - Dermal, Category 4

Acute toxicity - Inhalation, Category 4

Hazardous to the aquatic environment, long-term (Chronic) - Category Chronic 3

2.2 GHS label elements, including precautionary statements

Signal word


Hazard statement(s)

H302 Harmful if swallowed

H312 Harmful in contact with skin

H332 Harmful if inhaled

H412 Harmful to aquatic life with long lasting effects

Precautionary statement(s)

P264 Wash ... thoroughly after handling.

P270 Do not eat, drink or smoke when using this product.

P280 Wear protective gloves/protective clothing/eye protection/face protection.

P261 Avoid breathing dust/fume/gas/mist/vapours/spray.

P271 Use only outdoors or in a well-ventilated area.

P273 Avoid release to the environment.


P301+P312 IF SWALLOWED: Call a POISON CENTER/doctor/\u2026if you feel unwell.

P330 Rinse mouth.

P302+P352 IF ON SKIN: Wash with plenty of water/...

P312 Call a POISON CENTER/doctor/\u2026if you feel unwell.

P321 Specific treatment (see ... on this label).

P362+P364 Take off contaminated clothing and wash it before reuse.

P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.




P501 Dispose of contents/container to ...

2.3 Other hazards which do not result in classification


9. Other Information
9.0 Usage
Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid. It is a chaotropic agent, mainly involved in the extraction of DNA and RNA. It is utilized to deactivate the influenza virus.
9.1 Usage
Guanidine thiocyanate is used for RNA isolation and can solubilize proteins. When the compound is used to solubilize proteins it can be removed by dialysis against urea without difficulty. The hybridization of guanidine thiocyanate-solubilize hepatocytes can be used as a method to quantify mRNA from hepatocytes. Blood sample lysate stored in guanidine thiocyanate has been shown to maintain nucleic acid integrity. Research describes guanidine thiocyanate to be ineffective for RNA isolation of plant tissue with a high levels of secondary metabolites and storage proteins.
10. Computational chemical data
  • Molecular Weight:118.16084g/mol
  • Molecular Formula:C2H6N4S
  • Compound Is Canonicalized:True
  • XLogP3-AA:
  • Exact Mass:118.03131738
  • Monoisotopic Mass:118.03131738
  • Complexity:57.6
  • Rotatable Bond Count:0
  • Hydrogen Bond Donor Count:4
  • Hydrogen Bond Acceptor Count:3
  • Topological Polar Surface Area:101
  • Heavy Atom Count:7
  • Defined Atom Stereocenter Count:0
  • Undefined Atom Stereocenter Count:0
  • Defined Bond Stereocenter Count:0
  • Undefined Bond Stereocenter Count:0
  • Isotope Atom Count:0
  • Covalently-Bonded Unit Count:2
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